Gru and Lucy and their girls—Margo, Edith and Agnes—welcome a new member to the Gru family, Gru Jr., who is intent on tormenting his dad. Gru also faces a new nemesis in Maxime Le Mal and his femme fatale girlfriend Valentina, forcing the family to go on the run.

A fanboy of a supervillain supergroup known as the Vicious 6, Gru hatches a plan to become evil enough to join them, with the backup of his followers, the Minions.